Cell Isolation
General Sample Processing Considerations
Sensitive cell types (i.e. Digested sample, DCs, tumour samples)
–Avoid harsh treatment to cells (i.e. Passing through tubing/column at high speed)
–Do not over-digest tissue samples.
–Use high quality enzymes and protocols
Previously Frozen samples
–Thaw cells quickly in a water bath – and wash out freezing media as quickly as possible
–Assess viability after thaw
–Use DNAse to reduce cell clumpiness