Cell Isolation

General Sample Processing Considerations

Sensitive cell types (i.e. Digested sample, DCs, tumour samples)

–Avoid harsh treatment to cells (i.e. Passing through tubing/column at high speed)

–Do not over-digest tissue samples.

–Use high quality enzymes and protocols



Previously Frozen samples

–Thaw cells quickly in a water bath – and wash out freezing media as quickly as possible

–Assess viability after thaw

–Use DNAse to reduce cell clumpiness